Instrument: Ion Torrent Proton
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA from human macrophages was extracted with the help of a kit (RNeasy Plus Mini Kit) as per the manufacturer's instructions. The extraction was performed immediately following the 12- and 96-hours infection period. The 'gDNA eliminator' column included in the kit was used to remove genomic DNA in all samples. For each experiment, RNA quantity and quality was measured using Agilent 2100 Bioanalyzer. The RNA with a high RNA integrity Number (RIN) (≥ 9) was used for Ampliseq and quantitative real time qPCR experiments. Total RNA was extracted and frozen immediately at -800C until Ampliseq is performed Total RNA (100ng) was reverced transcribed using the SuperScript® VILO™ cDNA Kit to generate cDNA. The target regions were amplified for 10 reaction cycles using the Human Gene Expression Core Panel. After partial digestion, barcode adaptors (from the IonCde Adapters Kit) were ligated and the libraries were purified with Agencourt ™ AMPure™ XP reagent and eluted in low TE buffer